SARS-CoV-2 (Covid-19) appears to be a man made chimeric virus

June 17, 2020

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[2016] SARS-like WIV1-CoV poised for human emergence
Proceedings of the National Academy of Sciences ^

| March 15, 2016
| Peter Palese, Icahn School of Medicine at Mount Sinai
Posted on 06/17/2020 12:03:38 AM PDT by Grandpa Drudge

From the first paragraph of this article:“This manuscript describes efforts to extend surveillance beyond sequence analysis, constructing chimeric and full-length zoonotic coronaviruses to evaluate emergence potential. Focusing on SARS-like virus sequences isolated from Chinese horseshoe bats, the results indicate a significant threat posed by WIV1-CoV. Both full-length and chimeric WIV1-CoV readily replicated efficiently in human airway cultures and in vivo, suggesting capability of direct transmission to humans.”And from the results summary in this article:Using the SARS-CoV infectious clone as a template (7), we designed and synthesized a full-length infectious clone of WIV1-CoV consisting of six plasmids that could be enzymatically cut, ligated together, and electroporated into cells to rescue replication competent progeny virions (Fig. S1A). In addition to the full-length clone, we also produced WIV1-CoV chimeric virus that replaced the SARS spike with the WIV1 spike within the mouse-adapted backbone (WIV1-MA15, Fig. S1B). WIV1-MA15 incorporates the original binding and entry capabilities of WIV1-CoV, but maintains the backbone changes to mouse-adapted SARS-CoV. Importantly, WIV1-MA15 does not incorporate the Y436H mutation in spike that is required for SARS-MA15 pathogenesis (8). Following electroporation into Vero cells, robust stock titers were recovered from both chimeric WIV1-MA15 and WIV1-CoV.

(Excerpt) Read more at pnas.org …

TOPICS: Conspiracy; Health/Medicine; Science; SocietyKEYWORDS: 20200517; ccd; covid19; niaid; pandemic

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SARS-CoV-2 (Covid-19) appears to be a man made chimeric virusHere is the blueprint for Modifying (man made) SARS-Cov based VirusPublished in PNAS (Proceedings of the National Academy of Sciences of the United Strates of America) March 14, 2016https://www.pnas.org/content/113/11/3048SARS-like WIV1-CoV poised for human emergenceFrom the first paragraph of this article:“This manuscript describes efforts to extend surveillance beyond sequence analysis, constructing chimeric and full-length zoonotic coronaviruses to evaluate emergence potential. Focusing on SARS-like virus sequences isolated from Chinese horseshoe bats, the results indicate a significant threat posed by WIV1-CoV. Both full-length and chimeric WIV1-CoV readily replicated efficiently in human airway cultures and in vivo, suggesting capability of direct transmission to humans.”And from the results summary in this article:Using the SARS-CoV infectious clone as a template (7), we designed and synthesized a full-length infectious clone of WIV1-CoV consisting of six plasmids that could be enzymatically cut, ligated together, and electroporated into cells to rescue replication competent progeny virions (Fig. S1A). In addition to the full-length clone, we also produced WIV1-CoV chimeric virus that replaced the SARS spike with the WIV1 spike within the mouse-adapted backbone (WIV1-MA15, Fig. S1B). WIV1-MA15 incorporates the original binding and entry capabilities of WIV1-CoV, but maintains the backbone changes to mouse-adapted SARS-CoV. Importantly, WIV1-MA15 does not incorporate the Y436H mutation in spike that is required for SARS-MA15 pathogenesis (8). Following electroporation into Vero cells, robust stock titers were recovered from both chimeric WIV1-MA15 and WIV1-CoV.And the connection to Wuhan Labs in China is highlighted in the Acknowledgments:We thank Dr. Zhengli-Li Shi of the Wuhan Institute of Virology for access to bat CoV sequences and plasmid of WIV1-CoV spike protein. Research was supported by the National Institute of Allergy and Infectious Disease and the National Institute of Aging of the NIH under Awards U19AI109761 and U19AI107810 (to R.S.B.), AI1085524 (to W.A.M.), and F32AI102561 and K99AG049092 (to V.D.M.). Human airway epithelial cell cultures were supported by the National Institute of Diabetes and Digestive and Kidney Disease under Award NIH DK065988 (to S.H.R.). Support for the generation of the mice expressing human ACE2 was provided by NIH Grants AI076159 and AI079521 (to A.C.S.).And an interesting side note: National Institute of Allergy and Infectious Disease (NIAID) is managed by Dr. Anthony Fauci, in case you were wondering if he had any connection to this.My assessment is that the PNAS article indicates strongly that NIH National Institute of Allergy and Infectious Disease, managed br Dr. Anthony Fauci, and the Wuhan Institute of Virology, managed by Dr. Zhengli-Li Shi cooperated to create what ultimately became the chimeric virus SARS-CoV-2 (Covid-19) pandemic.https://www.pnas.org/content/113/11/3048My further assessment is that the gop-foreignaffairs report is investigating CPP for malfeasence, but apparently not interested in understanding where this virus originated, they are only interested in blaming CCP for the cover up.https://gop-foreignaffairs.house.gov/wp-content/uploads/2020/06/Interim-Minority-Report-on-the-Origins-of-the-COVID-19-Global-Pandemic-Including-the-Roles-of-the-CCP-and-WHO-6.15.20.pdf

To: Grandpa Drudge

2
posted on 06/17/2020 12:17:22 AM PDT
by M Kehoe
(DRAIN THE SWAMP! Finish THE WALL!)

To: Grandpa Drudge

“Chimeric virus”…I am reminded of “Chimera” in MISSION IMPOSSIBLE 2…It was a biological weapon…
MI 2 was a good action film…

3
posted on 06/17/2020 12:18:37 AM PDT
by L.A.Justice

To: Grandpa Drudge

There was an article posted to free republic maybe a month ago, from a scientist saying that this sort of thing was well known in the scientific community -— that they had been researching this modification of corona viruses…
But for whatever reason the press isn’t reporting it.
Yeah… it isn’t conspiracy talk any more

4
posted on 06/17/2020 12:33:24 AM PDT
by Mount Athos

To: Mount Athos

If it waddles like Peking duck…

5
posted on 06/17/2020 12:44:05 AM PDT
by CharlesOConnell
(CharlesOConnell)

To: Grandpa Drudge

Title is:
SARS-like WIV1-CoV poised for human emergence
Not the title you gave it.

6
posted on 06/17/2020 12:44:09 AM PDT
by ifinnegan
(Democrats kill babies and harvest their organs to sell)

To: Grandpa Drudge

You don’t know anything about molecular biology or bioinformatics, do you?

7
posted on 06/17/2020 12:48:40 AM PDT
by ifinnegan
(Democrats kill babies and harvest their organs to sell)

To: Grandpa Drudge

“SARS-CoV-2 (Covid-19) appears to be a man made chimeric virus”
I don’t see anything in the paper saying that.
In fact it expressly says that SARS-CoV likely emerged from Chinese horseshoe bats, and that similar viruses living in that bat population could emerge as future SARS outbreaks.

8
posted on 06/17/2020 12:57:20 AM PDT
by Pelham
( Mary McCord, Sally Yates and Michael Atkinson all belong in prison.)

To: Grandpa Drudge

The paper details stupid virology tricks, but does not say covid 19 was man-made.

9
posted on 06/17/2020 1:12:37 AM PDT
by Brooklyn Attitude
(In America 2.0, blacks are sacred and can do no wrong.)

To: Grandpa Drudge

Posting fake headlines especially while a noob is not a promising way start on Free Republic.

10
posted on 06/17/2020 1:26:09 AM PDT
by Brooklyn Attitude
(In America 2.0, blacks are sacred and can do no wrong.)

To: Brooklyn Attitude

The Chinese have a cure. Make them give it to us.

11
posted on 06/17/2020 1:38:51 AM PDT
by abbastanza

To: Grandpa Drudge

No, the linked article does not confirm that the National Academy of Sciences has determined that COVID-19 was man-made. DON’T POST LIES.

12
posted on 06/17/2020 1:39:19 AM PDT
by TChad
(The MSM, having nuked its own credibility, is now bombing the rubble.)

To: abbastanza

“The Chinese have a cure. Make them give it to us.”
How do you propose to do that? Trying to use force would be worse than the disease.

13
posted on 06/17/2020 1:50:23 AM PDT
by Brooklyn Attitude
(In America 2.0, blacks are sacred and can do no wrong.)

To: ifinnegan

Re: “Not the title you gave it.”
Besides the harmless “[2016],” the title looks exact to me.

14
posted on 06/17/2020 2:18:53 AM PDT
by zeestephen

To: Grandpa Drudge

Looks like you stirred up a severely concerned set of “Karens”…
First for kindly letting us know it was a 2016 article,
then for making some reasonable observations,
and for highlighting connections contained within.
You must be over the target.
Thanks for posting!

15
posted on 06/17/2020 2:50:03 AM PDT
by EasySt
(Say not this is the truth, but so it seems to me to be, as I see this thing I think I see #KAG!)

To: TChad; Brooklyn Attitude

Re: “DON’T POST LIES.” Comment #1 is not accurate. However, links to this paper have been posted several times at Free Republic, and it does contain important information. The Wuhan Lab, and specifically its director, were attempting to modify the bat virus into a form that would infect humans, a form that could be studied in the lab before a natural transition might take place. The Wuhan Lab director’s work was funded by NIAID, where Dr. Fauci is the director. Presumably, Dr. Fauci approved the Wuhan funding. Since COVID-19 was first identified at an open market place within walking distance of the Wuhan Lab, and since Dr. Fauci was a top USA government executive involved in the COVID-19 crisis, that is definitely a coincidence worth investigating. Naturally, none of the mainstream press will investigate, so the amateurs at Free Republic – like me – have to stumble around on our own.

16
posted on 06/17/2020 3:03:55 AM PDT
by zeestephen

To: zeestephen

“Besides the harmless “[2016],” the title looks exact to me.”
Admins changed it to the correct title from the listed fake title.
It was initially posted as “SARS-CoV-2 (Covid-19) appears to be a man made chimeric virus”

17
posted on 06/17/2020 8:34:12 AM PDT
by ifinnegan
(Democrats kill babies and harvest their organs to sell)

To: ifinnegan

SARS-CoV-2 (Covid-19) appears to be a man made chimeric virus is the title I intended to use. The title that appears is the title of the PNAS article that serves as the basis of this claim. I am not an expert at FR posts, and don’t know why FR used the article title rather than the title I specified.

18
posted on 06/17/2020 9:50:27 AM PDT
by Grandpa Drudge
(Just an old man, desperate to preserve our great country for my grandchildren.)

To: ifinnegan

Looks the same to me, with the helpful date flag.

19
posted on 06/17/2020 10:08:04 AM PDT
by citizen
(Women are from Venus and Men are from Mars. A,ll the other genders you make up are from Uranus.)

To: ifinnegan

You ask “You don’t know anything about molecular biology or bioinformatics, do you?” I’m certainly not expert in those fields, but I DO know how to read. Do you? There is plenty of plainly stated information in that Proceedings of the National Academy of Sciences article to support my assessment.

20
posted on 06/17/2020 10:13:56 AM PDT
by Grandpa Drudge
(Just an old man, desperate to preserve our great country for my grandchildren.)

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